Eun+Ji

Title
what is the best condition for mold(bacteria) to grow?

Goal
To find out the facts that influence on the growth of mold(bacteria).

Hypothesis
The rice which is placed in __wet, uncovered and dark place__ gets more mold than the others having different conditions. The mold growing on the rice would be a kind of __red mold.__

quantity of sunlight quantity of air quantity of water (humidity)

the growth of mold (cm or square cm)

Variables held constant
amount of the rice (5g each)

Materials
40g of rice water spray 8 petridishes black clothes a ruler

Process
1. Prepare all the materials. 2. Find the place where lets the sun in. 3. Set up the 8 chalets having diffent conditions. 4. To make the rice of four petridishes wet, spray water but not too much upon the rice with a water spray. But do not give a change to the other four. - To keep being wet, you have to spray the water once a day. 5. To give a difference on the amount of air, four patridishes are covered with the lid but the other four are not. 6. To give a difference on the amount of sunlight, four patridishes are covered by the black cloth to block the sunlight but the other four do not have any changes 7. Label all the petridishes in the order having different condition each. __a) covered/wet/bright b) covered/dried/bright c) covered/wet/dark d) covered/dried/dark e) uncovered/wet/bright f) uncovered/dried/bright g) uncovered/wet/dark h) uncovered/dried/dark__ 8. Check the change of growing mold everyday and for the four patridishes which has to be wet, spray the water once a day. You can check how much the mold grows by its size measured by a ruler. 9. Write down or roughly draw the results.

Expectaion
My partner(Felis) and I don't have that much time and this experiment is kind of taking long time one so what is the most important thing to get the research(find the mod) as soon as possible is managing the given conditions of each petridish and checking the growth of mold everyday. I expect the result can come out as I guessed in my hypothesis.

Result/Data

 * day/growh of mold(cm) || patridish a) || b) || c) || d) || e) || f) || g) || h) ||
 * 1 ||  ||   ||   ||   ||   ||   ||   ||   ||
 * 2 ||  ||   ||   ||   ||   ||   ||   ||   ||
 * 3 ||  ||   ||   ||   ||   ||   ||   ||   ||
 * 5 ||  ||   ||   ||   ||   ||   ||   ||   ||
 * 6 ||  ||   ||   ||   ||   ||   ||   ||   ||
 * 7 ||  ||   ||   ||   ||   ||   ||   ||   ||
 * 7 ||  ||   ||   ||   ||   ||   ||   ||   ||

I set up the experiment of mold on Thurstday of May 17th and the following day of May 18th there was no mold found on the rice. However, during the weekend there were a lot of changes. On Monday when I check the padridishes, every padridish execpt F(uncovered/dried/bright) gets various types of mold. My partner and I didn't expect that the mold would grow like this big and a lot. We were so surprised when we firstly saw the molds and initailly we reached close to the petridishes it was really smelly and disgusting. According to my hypothesis I mentioned that Petridish G (uncovered/wet/dark) would get the mold the most with red mold but not really. As there are too many species it is really hard to measure and actually I planned to measure the molds by ruler in suqare cm or something but it's impossible.

Petridish C is also really damp. Blue, black, yellow and brown colored molds are shown but not spreaded too much. Petridish D is not moist at all. Half of it is covered with whitish mold and the other part is covered with dark-yellow, dark-green and black colored molds. Like Petridish B, it also gets fiber-like stuff. Petridish G. One part of the rice gets yellow and green color combined mold and the other part has grayish mold with fiber-like thing. It jas beem given water but not that wet like patridish A or C because it has been under the sunlight. Petridish H does not get much mold. In the middle of the rice gets black colored and fiber-like mold. It's really hard and dried. All the shown molds have different colors and figures. Petridish A,B and D have the molds the most. However some of them are also wet although they were not given water. . Petridishes A B C D A B C D E F G H E F G H

Petridish A is so humid that I cannot not open the cover easily because of the dampness, the cover is sticked on to the rice. Generally the rice becomes yellowish and some parts get black or dark-green colored mold. Petridish B is really really moldy. Middle of the rice gets grayish and dark greenish mold. On the side, there are yellow, brown and white cololored molds. And white and thin stuff like cotton or fiber covers the rice overally.

Petridish E is really hard because it has been under the sunlight(patridish F also). The rice becomes really colorful with gray, yellow, black, purple. orange and green colored molds. Petridish F is the only thing which does not get mold. It has no change and just dried.

- the growth of the mold of rice is an independet variable of my experiment. - the amount of air, sunlight and water are the dependent variables of my experiment. The colors of molds are due to spores borne on the filaments. Most molds are saprophytes. Some species (e.g., penicillium) are used in making cheese and antibiotics. - a lot of bacteria are found in the mold. - mold produces more spores in humid, dark and opened environment. - one of the most well known red mold is monascus, a kind of yeast. - red mold is a genus of mold
 * Independent** **Variable** - factor in an experiment that a scientist purposely changes; also known as a responding variable.
 * Dependent Variable -** factor in an experiment that a scientist wants to observe, which may change becuase of the manipulated variable; also known as a responding variable
 * Mold** - multicellular organism of the division Fungi, typified by plant bodies composed of a network of cottony filaments.
 * Bacteria** - very small organisms. Some bacteria can cause disease.
 * Spore** - a cell produced by bacteria and fungi which can develop into new bacteria or fungi.
 * Monascus** - a genus of mold which is used in the production of certain fermented foods in East Asia.
 * Zygomycetes** -
 * Genus** - a class of similar things, especially a group of animals or plants that includes several closely related species
 * gametangium** - gamete-producing structure found in mold

Appendix 2 (Key Concepts, Models, Big Ideas)
-Text Book 470pg~542pg

- Bacteria - Mold
 * http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/E/Eubacteria.html
 * http://fig.cox.miami.edu/Faculty/Dana/monera.html
 * http://www.epi.state.nc.us/epi/oii/mold/grow.html
 * http://nethack.wikia.com/wiki/Red_mold
 * (red mold) http://fig.cox.miami.edu/Faculty/Dana/monera.html

- the life cycle of the zygomycete



- structure of Monascus

- the mold magnified by microscope